FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_positive-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_positive-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8549276
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCCC35050.041.570404145
CTCGGAG32500.037.02557
TCTCGGG48100.032.251125145
GCTCGGA36550.025.3861166
TCGGAGA46400.025.3088998
CGGAGAT68550.024.639099
TACACTG30550.019.2217645
CTATACT29100.018.1869324
CGGTGGG94300.017.987896145
CGTGTAA49850.017.013628145
CTAGACT9250.016.4591394
GACCCCC32750.016.379349145
CTACCCT21350.016.299454
TATACTG44550.015.9476895
TCTATAC32300.015.9364093
TCTACAC34100.015.7330153
TACACCG56500.015.2692575
TATACAG50100.015.0492545
GTATAGG26500.015.0469661
TATACCG44300.014.8921395
Produced by FastQC (version 0.11.5)