FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_neg.8.2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_neg.8.2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGCTCGGAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTG160.5854372484449323No Hit
CGTGGGCTCGGAGATGTGTATAAGAGCTGTCTCTTATACACATCTGACGC50.18294914013904134No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT100.006706628145.09
TCGGAGA100.006706628145.08
CGTGGGC100.006706628145.01
GTGGGCT100.006706628145.02
GCTCGGA100.006706628145.06
GGCTCGG100.006706628145.05
CTCGGAG100.006706628145.07
CAAAACC100.006706628145.01
Produced by FastQC (version 0.11.5)