FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_8724.tr6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_8724.tr6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9231708
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT58300.084.552279
GCTCGGA35150.080.0173656
CTCGGAG52850.075.1650167
TCGGAGA52300.074.708038
GGCTCGG33750.052.1978575
GGGCTCG28350.047.3094864
TCTCGGG70150.046.294872145
ACTCGGA20900.037.4589276
CGACGAA61850.035.86446145
CGGTGGG97450.035.408512145
TGACGCC38100.034.628124145
CGTGGGC33000.027.461591
TCTCGGA22550.027.002916
TCGCCGG141250.025.814402145
TGCTCGG31650.024.5092625
AGCTCGG26900.023.1774885
CACGGAG27550.022.8916917
TAGCTCG14300.022.307264
ACGATAA60850.021.6817145
CCCGGAG20150.021.5852177
Produced by FastQC (version 0.11.5)