FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_8724.tr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_8724.tr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGAACAGTCCGGTGTTTTTATCTGTTATCTGTGACTTAAAACTAAG14.545454545454546No Hit
CCTTTTATCAAGGTTCTTTGATTCTTTTCATTGGATTAGAACATGCTCCT14.545454545454546No Hit
GTTCTATTCCATTAATTCCTTTTAATAAGAATAAAAGTTTTAATACTTCT14.545454545454546No Hit
GTATTACATATACAGAGCAGGGTTAATCTGGCTGGGGGAAAAGAGTCTTA14.545454545454546No Hit
AATGCCTCGCCACCTGCGATGGTTCCGTTCACCGCAACACCTGCCACCGT14.545454545454546No Hit
AAAACATACCGACCCTTGGTCTAGAGTACTTTGGAAGCTCCCTAATTTCA14.545454545454546No Hit
GGGCAGAGGCTAGAGAAAAACAAAGCCCTTTCCTACTTACAAGCTTTGAG14.545454545454546No Hit
TTGTGAAAAGCTCAAAGGCTATGGGAAGTGCAGCCTCCATAAAATAAAAT14.545454545454546No Hit
GTTTTAATCTCTCGTAGTTCATTGAGTTTATCCTCTATATAGTTCATCTG14.545454545454546No Hit
GACCAAGGGGCCAAAAGAACTGGTGGCTGGTGAAGGCCAACTTCCTGGCC14.545454545454546No Hit
CCTCTTTTACGCTGCTTGTAACTGTTGAAGGGCTTCTAAATATATGTTTG14.545454545454546No Hit
TTTGAATTCCATTTGATGACAATTCCATTCAATACCAATTGATGATGGTT14.545454545454546No Hit
CAGTAGCGGGGTTGTGGGTTGGGGTCCGAGCTGCGGTGCGCTCATCTTTT14.545454545454546No Hit
CTTATGGGGTAAACTGAGACATTCCAGGAGCCTGGAATCAATGTGAAGTA14.545454545454546No Hit
GAACAAAACACTGACTCAAAAAAAAAAAAAAAAAAAAAATTTCCACCCTA14.545454545454546No Hit
GTACAGGCCCAGCTGGAAGCGGCGCGCCTGCATGTCCGCGGCCTTCGGAC14.545454545454546No Hit
GAACCTATGGGAGGAGCATAGGGTAACGTTGACAAGTACGGTCGGCGGAT14.545454545454546No Hit
GATTGGATCTGCTTTATTAGTATCTGGCGCCATTGGTAATGGTATCGATC14.545454545454546No Hit
ACGCAGCAGAGAGCGGCGAGAGAAAGACTGAGAAAAATAAGACATAAGAG14.545454545454546No Hit
AACTGAAACAGGTAAGGAGGCAGCTTGAGGCTACACTTGATTTGACACCA14.545454545454546No Hit
GGTTTGTCCAGCATGTACCGCTACGAAAACGTTATTCCATTGTGTAATGC14.545454545454546No Hit
GGAGAAATACAGCACAAGGTTACAATAACCAAAGATTTCTATATGAGTAA14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers