FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_8724.tr3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_8724.tr3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10104237
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT40800.076.578759
TCGGAGA34850.072.595838
GCTCGGA24800.071.90656
CTCGGAG39250.067.412787
TCTCGGG67650.049.935486145
GACGAAA73050.046.144924145
GGCTCGG22650.043.210045
TGACGCC30650.040.444252145
GGGCTCG18500.035.269614
ACTCGGA20350.033.4848376
CGGTGGG87050.033.310596145
TGCTCGG20350.028.143745
TCGCCGG131550.027.938835145
TAGCTCG11250.020.6218364
TCTCGGA20750.020.6119296
CGTGGGC26900.020.2134761
AGTGTAA135050.019.37777145
AGCTCGG22650.019.2044625
GCACGGA16450.018.9490436
CGCGGAG11750.018.508557
Produced by FastQC (version 0.11.5)