FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_8337.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_8337.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11174850
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT48800.089.573419
TCGGAGA38550.084.807038
GCTCGGA30200.084.251926
CTCGGAG46050.072.411557
GGCTCGG30550.054.1068045
TGACGCC32300.049.599045145
GGGCTCG24900.046.0040134
TCTCGGG88650.042.521458145
GACTCCC75700.040.411053145
TGCTCGG23250.032.429385
CGCGGAG13200.032.4009487
CGGTGGG121900.032.171932145
ACGTGTT38950.031.825277145
GCGCGGA11200.030.4200216
GCCGACC29400.030.327831145
ACTCGGA20000.030.0834566
CGTGGGC30950.029.9841121
CGTGTAA82500.029.435703145
GTCGCCC53400.029.322166145
GCACGGA16150.028.2779356
Produced by FastQC (version 0.11.5)