FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_7784.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_7784.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9599130
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT39700.047.1097459
TCTCGGG63350.044.283905145
TCGGAGA39650.036.5652358
GCTCGGA30900.035.8930176
CTCGGAG46150.032.2005847
GACGAAA80100.030.951033145
CGGTGGG81750.027.134087145
GGCTCGG31000.020.3460395
TCGCCGG127700.020.095272145
TGACGCC45000.019.33082145
AGTGTAA113700.016.89534145
GGGCTCG27050.016.8853134
GTATTAG56200.015.0933411
TATACTG77850.014.8067855
TAGACAG87650.014.8055115
GTCCTAG27200.014.3933111
GTATAGG33800.014.371231
GCCGTAA85850.014.3545685145
TACACTG60050.014.0044885
CTAGTAC24500.013.9080933
Produced by FastQC (version 0.11.5)