FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_7379.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_7379.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10173961
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT45200.062.22639
TCGGAGA35150.059.3947228
GCTCGGA29850.057.5543676
ACGATCC25850.045.98961145
CTCGGAG51800.041.8427287
TTACGCC24600.037.718227145
TGACGCC38200.034.91651145
CGACGAA161400.027.486809145
GGGCTCG30100.026.9758364
GGCTCGG38800.026.7191965
GCCGACC31200.025.324934145
CGCGGAG20200.022.608297
GCGTGTT88250.021.603168145
GCGCGGA18350.020.1469086
TCTTACC72200.020.080256145
TACGCGG11900.018.883877145
GCACGGA21550.017.828016
ACGAACC20800.017.773922145
TTGCTCG20050.016.6328564
ACTCGGA21800.016.293486
Produced by FastQC (version 0.11.5)