FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_341.tr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_341.tr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATAACTTCATCAGGTAGAATCATTGAGAGCAATTGTCTCTGAACTGTC11.8867924528301887No Hit
AACAAAAAATTAAACCAGCCCGGCACGGGAAACCCCACTTAGCTCCGTAA11.8867924528301887No Hit
AATACAATAACTCATGACACCCACGAACACCCCCCACCCCCCCCACCCCC11.8867924528301887No Hit
AGGTTACTCACTTAGCTAATGGCAGAACCAGAACTAGAATCTAGAACTTT11.8867924528301887No Hit
GCCTAGGCTGGTCTTGAACTCCTGGCTTCAGGATATCCGCCCATCTTGGC11.8867924528301887No Hit
GAAAGAATATGAGAAAAGAAGGAAGAAAAGGGAGGGAGAAAGGAAGGGAG11.8867924528301887No Hit
CCGCCAAGGTTGCTTTTTTTTTTTTTGAGACAGAGTCTCCCTCCTGTCTC11.8867924528301887No Hit
GTCCTCACAGTAACCCTGTGGGGTAAATGCTGTTATCATGTGACAGATGA11.8867924528301887No Hit
TTACAGGCTTGTGCCACCACAACCGGCTAATTTTTATATTTTTAGTAGAG11.8867924528301887No Hit
TCAGGATCCAGAGAGGGCCCATGCATTGTATTTGACTATATGAGGCTCTT11.8867924528301887No Hit
TTTAAAGCCTGAGCAACATAGCAAGACCCCATCTTTCCATGAAAGTTTTT11.8867924528301887No Hit
ATCGCAAGGTTAGGAACGGATAGACCTTTCTTCTCTGTGGACGACTTGCG11.8867924528301887No Hit
GTAACCTGCACTCGATGGGGGGAGGGGTGAATTGTAAGAAAATACACCAA11.8867924528301887No Hit
GTCTATGCATAAGTTTCAGACTTATTCCCAAGTTTTGGGTTTTTTTTTTT11.8867924528301887No Hit
CAGGATACCATTCTCAGCTTGGGACTTTCAACTGATGGCTGAGCTGAGAG11.8867924528301887No Hit
ATGTAATGGCCTTCTTTGTCTCTTTTGATCTTTGTTGGTTTAAAGTCTGT11.8867924528301887No Hit
ATATATATATATATATATATATATGTATAAATATGTTGGGTTTTTTTTTT11.8867924528301887No Hit
GTCTTTGTTCTCATTGGTTTCAAAGAACATCTTTATTTCTGCCTTCATTT11.8867924528301887No Hit
GTCTCGAACTCCCAGCCTCAGGTGATCTGCCCGCCTTATCCTCCAAAAGT11.8867924528301887No Hit
GTTTTTTCCAGTCCTGCGGGTGGAAGGCAGTGGTTGTTTCAGGAAGAATT11.8867924528301887No Hit
GCCTTTCTTTTTTTTTTTTTTTTTTTGAAACAGGTTTTTTCTCTTTTTCC11.8867924528301887No Hit
CACCTGTAATCCCAGCACTTTGGGAGCCTGAGGCGGCTGTCTCTTATACA11.8867924528301887No Hit
TTCTTTAATTGTATGCCACCTTTTTTTTTTTTTTTTTTTTTCTCTGAGAC11.8867924528301887No Hit
GAGTAATGATCCTGCTCTTTGACCAGTCCATCTAAAGTAATGGTCATAGT11.8867924528301887No Hit
TATTTTTAAAAGCCTTGATAAGTTACTTTCCAAAAGAACAAGTCAGGATT11.8867924528301887No Hit
GGATGTGAGAGCACAGACAAAGAGACCCACTTTTTTTTTTTTTTTTTTTT11.8867924528301887No Hit
CCAGTAAAGAGAGTATGACTTGTTGAGGGCTCAGATTATGATTGGCACTC11.8867924528301887No Hit
GTGAAAAGGACTTTTTTTTTTTTTTTTTAAAAAAAAAAAAACACACCCTT11.8867924528301887No Hit
ATTTTACATTGCCACCAGCAATGCCTGAGGATTCTCATTTCTCCACATCC11.8867924528301887No Hit
CCAGAGGGTAGCTGAAAAATGAGAAGGTAATATTATTAAATAATGTAGAA11.8867924528301887No Hit
CGCCCAGCCTTCTTCTTTTTTTTTTTTTTAGACGGAGTTTCGCTTTTATC11.8867924528301887No Hit
GTGCAAAGGAAAATAATCGAAGTAATAAAATCTAAGGTTTCATTTGTTTT11.8867924528301887No Hit
TGTGTGTGTTTTTTTTTTTTTTTTGAGATGGAGCCTTGCTGTATCGCCCA11.8867924528301887No Hit
GTGCGACCATTCGAACTGGAGGACAGAGGCGCCAGCCCCCGCGTTGTGCC11.8867924528301887No Hit
ATCTAGGACAGTCAAATTAGTATCCCTTTCCTCTAAGAGCTTTTCAATTC11.8867924528301887No Hit
CTTAAAAGGACATGATTTTTGTGTTTTTTTTTTTTTAACGTGCACGCAGT11.8867924528301887No Hit
CCTTAAGCTAGGGTCTGGGGACAGGTTTTATAGGCAGAGTGACTATGAGG11.8867924528301887No Hit
CCTCACAGGTGTGGGGCTGCTGGCCGTGGGGGCGGCCGCTTCAAAGCATC11.8867924528301887No Hit
GATTACAGGTGCCCGCCACCATGCCCAGCTAATTTTCTTTCTGGTTTTTT11.8867924528301887No Hit
GGGTATTGTTGGGTCCCGTGTAAAAAAAAGAGGCAGCTGAGTGGAAGCGG11.8867924528301887No Hit
ATAGAAAAAAGACCCTACTAAACCAGCATTTTATACAAATTATTATACAT11.8867924528301887No Hit
CTCTGGGGCCTCTTCTACAAGTTCACTAATCTCATTTGTTACGGCTCCAC11.8867924528301887No Hit
CAATTTATCATTGAATAAGTGTTTTAAGAAAGTGGACAAAGAGAGGAGTC11.8867924528301887No Hit
TCCTTTCATATTGGTGGTATTTGTTTTCAGTTTGTTTTTTTTTTATTTTT11.8867924528301887No Hit
CATTATAACTCACTCACTATCTTTTTTTTTTTTTTGAGACGGAGGCTTGC11.8867924528301887No Hit
GATCTATTGCATCCACTGAATCTCCTTATTGCATTCTAAACTAGGTTTTT11.8867924528301887No Hit
CCACCACGCTGGGCTTTTTTTTTTTTTTTTTAGTAAAAACAGGGTTTCAC11.8867924528301887No Hit
GAAGTAGCCAGCATGAATGTGGCATCCAAATCCCATAGAAATGGAATGGA11.8867924528301887No Hit
TGGTAACAGTGACACATCATTAATTAATGCACATTTTATTGGCTTTTCTC11.8867924528301887No Hit
CCACCACATACATCCACCTTATTCTTTTTTTTTTTTTTTTTTTTTTTGAG11.8867924528301887No Hit
GCTGTGAATGATAAGGAACAAGGGGAACATTTTACTTTTTTTTTTTTTTT11.8867924528301887No Hit
ACTCATAAATGCTTTTCCAAGGCTGATGACTAGAATGGTGTTTCCTAGTT11.8867924528301887No Hit
CTGTTCTCCACGTTGGAAATGAGAGGCTTTACTGCTCAGATGCTGCAAAA11.8867924528301887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCC50.0145.07
TTACATT50.0145.04
TACATTG50.0145.05
ACATTGC50.0145.06
TTTTACA50.0145.02
ATTGCCA50.0145.08
CTGCAAC50.0145.0145
TTTACAT50.0145.03
ATTTTAC50.0145.01
TTGCCAC50.0145.09
TTTTTTT400.00837586814.575-79
Produced by FastQC (version 0.11.5)