FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_341.tr1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_341.tr1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7551913
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT31500.037.740249
CTCGGAG30500.033.273147
TCGGAGA32400.031.3219388
GCTCGGA24850.030.9201936
TCTCGGG31300.029.181019145
GACGAAA49900.020.7735145
TACACCG26700.018.1918965
CGGTGGG45350.017.42302145
GTATAGG33950.016.6561661
TATACTG62900.016.3663625
CCTACAC35900.016.1553293
GGCTCGG26700.015.2051685
CTACACT41850.014.7246064
CTAGGAC25650.014.6972543
CTAATAC55550.014.4863563
TGACGCC30600.014.213598145
TACACTG49650.014.1633825
TGGACCG17250.013.86881355
GTGTAGG37150.013.8554221
TACACAG61250.013.7298675
Produced by FastQC (version 0.11.5)