FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_3256.tr4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_3256.tr4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9939845
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT32200.078.7938469
TCGGAGA29550.069.423578
GCTCGGA21100.068.023436
CTCGGAG38250.054.959727
TGACGCC23250.042.40274145
ACGATCC23200.039.68201145
GGCTCGG23350.037.8777055
CGACGAA116200.031.628584145
GGGCTCG19650.031.3604564
TTACGCC15900.031.002005145
GCCGACC18950.030.98516145
TGCTCGG19450.025.3454515
ACTCGGA17850.024.772366
GCGTGTT67750.021.82724145
TACGCGG6700.021.6388145
TAGCTCG8750.020.7137134
CGCGGAG9550.020.4945477
CACGGAG23700.020.4929457
GCACGGA15350.019.3620456
GCGCGGA8400.018.1224026
Produced by FastQC (version 0.11.5)