FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_30982.tr4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_30982.tr4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6843104
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG23350.037.5654347
CGGAGAT31800.032.8265539
TCGGAGA27650.031.7234298
GCTCGGA23400.025.0933726
TCTCGGG30300.018.900547145
TATACCG13650.018.590135
CCTATAC24750.018.162793
TACACTG46400.017.5003645
CTATACT39950.016.515344
TATACTG56800.016.083085
TACACCG23050.015.7270075
GTACTAG16650.015.6766881
TCTATAC39650.015.5432493
GTCTAGG24100.015.3433141
CCTACAC29600.014.9418443
CTACACT33150.014.6539314
TACACAG53200.014.1732265
GGCTCGG30450.014.0479125
GTATACG11950.013.9548771
GTGCTAG28650.013.6658051
Produced by FastQC (version 0.11.5)