FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_30900.tr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_30900.tr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAATTCGACATACATCTCTAGTGGCTTTTTTTTTTTTTTTTTAAACG13.225806451612903No Hit
GGTTCACCCTTCTCTGATCTCTGGCCCAGAAGCCTGCGTGAAAGTAAACC13.225806451612903No Hit
TCATTGTCACCTCCAGTCCAGGCACTCCACTTCCCTGGTCACACCCTGCA13.225806451612903No Hit
GAGCCACCGTGCCTGGCCAGTTTTTCCAATTATTTAGATGGTCTTTAATT13.225806451612903No Hit
GCCCGGCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAGGTTCAC13.225806451612903No Hit
GTGCAGTGGTGCAATCATTGCTTACTGCAGTTTTAACCTCCCAGGATCAA13.225806451612903No Hit
ACCCATAGGGTTATATGCTGTACATAATCTACTATTTCTCAAAGACTTTG13.225806451612903No Hit
CACCGTATCTCTGGGTCCAACTTTCTTTTTTTTTTTTTCCCCTCTCCCCC13.225806451612903No Hit
CATCTATTCCACCTTCTTTTTTTTTTTTTAACTTTTTAATAATGGCCATT13.225806451612903No Hit
TCCTTGGGGAGACACAGCTGGTCCACTGTTTTTTTTTTTTGTTGTTGTTT13.225806451612903No Hit
GACACTGCTTGCAGATAAATCAATCTGCTAGTCGGATTCTTTTTTTTTTT13.225806451612903No Hit
TTACAGATGTCAGCCACAGTGTCCGGCCATAGAATTTTTTTTTTTTTTTT13.225806451612903No Hit
GGTAGAATGTCTTGTCTTTCAAACTTATATTTGCTTTTTTTTTTTTTTTT13.225806451612903No Hit
GTGTCATCCCCAAATATATATTTATTTTATTCATGCATGGGATTTTTGGG13.225806451612903No Hit
CGGAGATGTGTATAAGAGACAGGCTTGAACCCAGGAGACAGAGGTTGCAG13.225806451612903No Hit
GGTGAGTTCTTATCCATTTGGCCCTTCTGTACCTTTTTTTTTTTTTTTTT13.225806451612903No Hit
AAATTATACCATAACATCCATAGCATCCTTTGAATATGTCTCAGATTTCC13.225806451612903No Hit
ATTCTGAATTTTTTTTTTTTTTTTGGAGACAGAGTCTCTGTGCAGGCTGA13.225806451612903No Hit
GGGGAATGGTGATTAACTTACCACCAATTATTTAGTATATCGAGAGCATG13.225806451612903No Hit
GGTATAAATCTTCATGACCTTTTTTTTTTTTTTAAACAATGGTTTCTTAC13.225806451612903No Hit
CTTTAATACTCTTGATATTTTTGTTAAGTGCATGTACTATTTTTTTTTTT13.225806451612903No Hit
AATTTAGATTTTGTCAGCTTTTGTTATTACTTTTTTTTTTTTTTTTTTTT13.225806451612903No Hit
CATATGTATGTGTATGTGTATATACAGATTACATGCCCGCAAATTCACAA13.225806451612903No Hit
CTGTTATGGTTATTATTTCTTTTTAAGTCTTTATCTTTTTTTTTTTTTTT13.225806451612903No Hit
GTGACTGGAACGTCGCCCCCGAAGACGCCGACGTGTGGGATATTGACTAC13.225806451612903No Hit
GTATTAACCCGAGGTCCCTATATCCCGCGTCTGCCCAGCCATCGTACCAG13.225806451612903No Hit
CCCATAGTTAATATCATAGGAATAGGGAAAAACTCAAAGTCTTTCCACTA13.225806451612903No Hit
TTCTACAACTGCAAGGCACCAACTTCTTCCTATGAAATAAATAGCATTGG13.225806451612903No Hit
GTTCAAGAGTAATTCAAGTATAATAAAAAAAACAAGAATAAATACAAAAG13.225806451612903No Hit
ACCATGCCCCGCTAATTTTTTTTTTTTTTTTTTTTTTTTTTAGTAGAGAC13.225806451612903No Hit
AGACAAAAGCCAATCTTAAAAGGTCACGTATTCCATTTCAGTTCTTTTTT13.225806451612903No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)