FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_MG_30900.tr3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_MG_30900.tr3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10624404
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT36300.038.742059
GCTCGGA25800.036.2451066
TCGGAGA36800.031.7147648
CTCGGAG41300.029.3122987
CGACGAA111750.028.736748145
TGACGCC40500.023.089584145
ACGATCC28650.022.7719145
GCGTGTT68900.019.25366145
TTACGCC29250.017.595995145
GGCTCGG33500.016.6635765
TACACTG76000.015.2626065
TATACTG73800.014.4405345
ACGCGTT124600.014.428297145
TATACCG14700.013.8090245
TACACCG21650.013.7292955
GTACTAG30350.013.3772851
GTATTAG71100.013.3579661
GTCCTAC34800.013.3333531
TGGACCG19100.013.2848595
ACTCGGA21850.013.2705196
Produced by FastQC (version 0.11.5)