FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924519
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG553270.9338648420234621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG3700.081.737897
GCTCGGA3800.077.696
TCGGAGA3950.072.918678
GGGCTCG5500.052.367984
GGCTCGG5700.050.5300875
CGGAGAT6100.047.2214139
GTGGGCT6750.042.673812
CGTGGGC8150.035.354681
TGGGCTC8850.033.359833
GTGTAAG3351.8189894E-1227.9539431
TACACTA3355.9240847E-821.494145
CTAGGAC2353.9131828E-521.4493123
GTATACT2704.7226386E-621.3351044
GTGTTAC3157.7590994E-720.5814741
AGTACTA2104.5216858E-420.5729625
CAGTACA3901.3225872E-820.3093764
GTTAGTA2857.5350326E-620.2139112
CTATACT3601.2666169E-720.0016614
TAGAACT5103.6379788E-1119.7663464
TATTACG1850.005250812519.4627172
Produced by FastQC (version 0.11.5)