FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_positive-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_positive-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8549276
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA31000.018.2503221
CTACACT22700.016.9268764
TCTACAC33250.016.7897263
TACACCG54050.015.961385
GTGTAAG27850.015.8870141
GTATTAG43200.015.7827161
TACACTG30400.015.500975
TATACTG42700.015.2803685
TATACCG43750.015.0793465
GTCCTAG10750.014.844031
GTATATA44550.014.8160121
CCTATAC23500.014.8087343
CTATACT27650.014.6831644
GTACTAG15850.014.6439541
TAGGGTG17200.013.909285
TTACACT38300.013.8181814
TATACAG50050.013.7606415
GTATAAG37200.013.6487461
CTACACA23450.013.603064
GTATACT28500.013.4821074
Produced by FastQC (version 0.11.5)