FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_neg.8.7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_neg.8.7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13739
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT374227.236334522163187No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC3552.583885290050222No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG3482.5329354392604992No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT1340.9753257151175486RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC570.41487735643059903No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT380.276584904287066RNA PCR Primer, Index 39 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC320.23291360361016086RNA PCR Primer, Index 39 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC270.19652085304607322No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT230.1674066525948031RNA PCR Primer, Index 39 (96% over 28bp)
CCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG210.15284955236916806No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGT200.14557100225635053No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACACCTCGTATGCCGT180.13101390203071547No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACC100.0070152474145.09
TCCAGAC100.0070152474145.03
TTATACA7200.082.569442
CTTATAC7250.082.01
TATACAC7400.080.337833
CACATCT7500.079.266667
TACACAT7550.078.741725
ACACATC7600.078.223696
CATCTCC7650.077.712429
ATACACA7650.077.712424
ACATCTC14250.041.71938
TCTTATA3152.7284841E-1127.6190471
CTCTTAT2609.829273E-825.0961531
CTTGAAA6500.022.75384755-59
GCTTGAA6500.022.5307755-59
TGCTTGA6550.022.35877855-59
TTCTGCT6650.022.240650-54
TCTTCTG6650.022.02255650-54
ATCTCGT6650.021.80451235-39
CCGTCTT6750.021.69629745-49
Produced by FastQC (version 0.11.5)