FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_neg.8.2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_neg.8.2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT136850.054884742041715No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG1324.829857299670691No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC1214.427369191364801No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT461.6831320892791803TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC160.5854372484449323No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGT140.5122575923893158No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT100.3658982802780827TruSeq Adapter, Index 10 (96% over 26bp)
CCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG80.29271862422246614No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT80.29271862422246614No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGT80.29271862422246614No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT70.2561287961946579No Hit
CTTATACACATCTCCGAGCACACGAGACCGAGGCTGATCTCGTATGCCGT60.21953896816684962No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC60.21953896816684962No Hit
CTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAG50.18294914013904134No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC50.18294914013904134No Hit
CTTATACACATCTCCGAGCCCACGAACCGAGGCTGATCTCGTATGCCGTC40.14635931211123307No Hit
CTTATACACATCTCCGAGCCCACAGACCGAGGCTGATCTCGTATGCCGTC40.14635931211123307No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG40.14635931211123307No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAAGCTGATCTCGTATGCCGT40.14635931211123307No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG40.14635931211123307No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG30.10976948408342481TruSeq Adapter, Index 10 (95% over 24bp)
TTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC30.10976948408342481No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.10976948408342481No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCG30.10976948408342481No Hit
CTTATACACATCTCCGAGCCCACGGACCGAGGCTGATCTCGTATGCCGTC30.10976948408342481No Hit
TCCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC30.10976948408342481No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGA100.006706628145.0145
CTTATAC2050.095.48781
CACATCT2100.093.2142947
TATACAC2100.093.2142943
CATCTCC2100.093.2142949
ACATCTC2100.093.2142948
TTATACA2100.093.2142942
TACACAT2100.093.2142945
ACACATC2150.091.046516
ATACACA2150.091.046514
CTCTTAT405.5332108E-572.51
TCTTATA658.277893E-655.769231
ACCGAGG2000.026.125-29
GACCGAG2000.026.125-29
AGACCGA1950.026.0256425-29
GGCTGAT1900.025.94736930-34
GATCTCG1900.025.94736935-39
ATCTCCG2100.025.5476210-14
CGAGCCC2100.025.5476215-19
TCTCCGA2100.025.5476210-14
Produced by FastQC (version 0.11.5)