FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_8724.tr6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_8724.tr6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9231708
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA20600.019.3557226
GGGCTCG19950.018.5333084
TACACTG54850.017.97535
GTGTAGG39900.016.3608931
TATACTG69200.016.238265
CTCGGAG24750.016.1102167
GGCTCGG23450.016.0758655
CGGAGAT25950.015.6446049
TACACCG21350.015.2801825
CTATACT51250.014.7117784
TCGGAGA27800.014.6035068
GTAGGAC22900.014.5633783
TTACACT63300.014.31633954
GCTAGAC28900.014.2993673
CTTACAC46300.014.2495183
TAGGACT44650.014.1261384
GTGTTAG49850.013.9682981
TATACAG65400.013.9671145
GTGTAGC38000.013.9340271
TTATACT85000.013.9025464
Produced by FastQC (version 0.11.5)