FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_8724.tr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_8724.tr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTACATACAATGACCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG14.545454545454546No Hit
GATTTAAAACAATGCTTGCTTTATCAAGTGGGAATATTGATGAAGTTATT14.545454545454546No Hit
GGCAAGAACCTTGCCTTAGTAGCTTTCATATTTCCGAGCCCACGAGACTA14.545454545454546No Hit
GTTCTGCACCTGGTAAACGAGCTGCATCAGATGTAGTAATAACTTTTGTG14.545454545454546No Hit
GGCATCATGCCCTAACTCGCACTCGGGTTAGGGCATGATCTGTTTTACTC14.545454545454546No Hit
TGACAATATATTCTCTTAACTTGCAATGTGGTTTTAGGAAACAATGAAAC14.545454545454546No Hit
TCGCTGCTCAACCAAGACGTCATCACTGTCAACAATGACGCCACCGGCAC14.545454545454546No Hit
GGGTGTGAGCCTGCGCGGCCGCATCGACGCGGTGTTCCGCACCGAGGATG14.545454545454546No Hit
GTTAAGCAGTCACCACATGACCCCACAGTCCCACTCCCAAGTATCTTCCC14.545454545454546No Hit
TGCCAGAACTGGTACTGGAAAGATTAAACATCTCGGAAGCATCTAACACT14.545454545454546No Hit
GTTCACACCATTGCCCTCTAGCCTGGGCAGCAAGAGCGAAACTCCGTCTC14.545454545454546No Hit
GCTTTGCTTCCTTGATGCTGTCACTTTTTTTTTTTTTTTTTTTTTTGGAA14.545454545454546No Hit
CAACAAAAGAGTCCCAACAAAAACCCCATCCAAGGGTTAGTAGCCTCAAA14.545454545454546No Hit
GAATAATTGCCCACTGCAGGATGAACAACCATACCTCGTTGTTTATTTAC14.545454545454546No Hit
GAAACATGGCATAATCTAAGCACATCTACCCTGGTTATTTTATTTTATGG14.545454545454546No Hit
GCCTCAGTGGGTCTGAATGCTGGAAATAAATCAACTTCAATTGCTGTTCT14.545454545454546No Hit
GTTCATAAGCCCTTGTATGTAGTACCTTCTAGTTTGTCTGCTCAATTTGG14.545454545454546No Hit
ATCGAATGTAATCATCATCAAATGGAATCAAAAATAACCATCATCAATTG14.545454545454546No Hit
CTCTAATTCCCAATGTGATGGTATCTGGAGGTGGGGCTTTTGGGAGGTGA14.545454545454546No Hit
TTTGGGGTCTGTCTGTTCGCCTTGTGGATAGTCATCGTACCAGTCGGCAC14.545454545454546No Hit
CCCTGGAGGTTAGAGGAATCAGTAAGAGATTTAGATGGCTCCATTATGAG14.545454545454546No Hit
GCTCATGCCAACAATCCCAGCACTTTGGGAGGCTGACACAGGAGGATTGC14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)