FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_8724.tr3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_8724.tr3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10104237
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA13050.026.1098636
AGACGCC29600.021.798807145
CGGAGAT17800.019.9569139
GGGCTCG16250.019.183894
TCGGAGA19000.018.6964768
GGCTCGG18100.018.4245345
CTCGGAG18900.018.0282387
TACACCG13050.017.7769285
GTATTAG64850.015.4341231
TATACTG90200.015.270895
CGTGGGC21450.015.2159161
GTGTTAG64250.015.0138261
GACTCCG32150.014.6571537
TACAGGT119900.014.0278264
TATACCG11450.013.9294645
GTCCTAG37500.013.9256061
TATACAG78750.013.6247465
TACACTG76100.013.5276065
CGTACAC12550.013.2866773
TCTATAC56850.013.2627983
Produced by FastQC (version 0.11.5)