FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_8337.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_8337.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11174850
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT16150.017.0583329
GCTCGGA14150.016.3952876
GTACCGT7601.8189894E-1216.2166376
TACACTG84900.015.4559755
TCGGAGA17200.015.1739818
GTGTTAC45300.015.0519991
TATACTG99000.015.0121945
GTATAGA64750.014.8996631
GTATTAG64350.014.8795551
GTATAAC41150.014.4546821
GGGCTCG17100.014.4149184
GTGTTAG75900.014.2399691
GTACGCC6702.6309863E-814.067643
GTACTAG29200.013.91136551
GTGTAAG62800.013.8607311
GTATAAG64900.013.6357711
GTGTAGG44400.013.5599991
TAGACAG125450.013.4651365
CTAGTAC31600.013.3074123
TATACGG8753.601599E-1013.2567615
Produced by FastQC (version 0.11.5)