FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_7784.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_7784.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9599130
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG32650.019.5501671
TAGACCG14200.015.8268165
GTATTAG60650.015.5476061
CTATACT57000.015.3897994
TACACTG61700.014.4524065
GTATAAG59000.014.38417151
TACACCG27150.014.1522745
TATACTG75150.014.0845845
CCTACAC45550.014.00688653
GTACTAG24400.013.9720141
GTGTAGG40200.013.8936281
GTATAGA56600.013.8407081
GTCTTAG45200.013.6405381
GTGTAAG50000.013.6366861
GTGTTAG55350.013.6290861
TATACCG15700.013.39117055
GTGCTAG33850.013.0714011
TAGAGGT58200.012.9548624
TATACAG68350.012.8341485
ATTAGAC44750.012.7991573
Produced by FastQC (version 0.11.5)