FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_7379.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_7379.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10173961
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA23050.024.2186
TCGGAGA23450.023.1865928
CGGAGAT27150.021.361849
GGGCTCG27800.019.297794
CTCGGAG32600.017.1234657
TACACCG11800.016.5882385
GGCTCGG34750.016.272655
GTCGTAG9800.015.54173851
TACACTG67850.015.2793435
GTATTAG48250.015.0317331
GTCCTAG47500.014.8110041
GTGTAGG46150.014.772791
GTGTAGA49200.013.85699751
TAAGGCG9155.0931703E-1113.4694074
CGTGGGC40700.013.3651311
GTATACG7609.475116E-913.3604411
GTATAGG27600.013.1391511
CTACACT59400.013.0592424
CTAGGAC49800.012.95688153
GTGTACG10753.6379788E-1212.8189221
Produced by FastQC (version 0.11.5)