FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_341.tr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_341.tr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACACCTGTAATCCCAGCACTTTGGGAGGCAGGTGGATCACCTGAGG11.8867924528301887No Hit
GATAGGAGGATTGCTTGAGCCTAGAAATTTGAGACCAGCCTGGGCAACAT11.8867924528301887No Hit
GGCTAACACAGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGG11.8867924528301887No Hit
TCCCAGCACTTTGGGAGGCCAAGGCGGGCGGATCACGAGGTCAGGAGATC11.8867924528301887No Hit
CAATAAAACGGATATAAGAGGATGTGTTAGCACATCCTCTTTTTTATTTA11.8867924528301887No Hit
ATCTCAAAGAGCGACTTAAGAGTAAAAAGAAACTTGAAATGGAGTCAGAT11.8867924528301887No Hit
GGGCGGATCACTTGAGGTCAGGAGTTCAAGGCCAGCCTGGCCAACATGGC11.8867924528301887No Hit
GTGGAGTATGGGGAACTGGGATAATTCTTCCTGAAACAACCACTGCCTTC11.8867924528301887No Hit
GGGAAAGGAAATTCTTTAAAATTATCTAAATGAATTAAATAGACTAAAAC11.8867924528301887No Hit
CAGTAACACTTAAGCACCATGACTATGACCATTACTTTAGATGGACTGGT11.8867924528301887No Hit
TTCAGAGACAATTGCTCTCAATGATTCTACCTGATGAAGTTATCTCTGTC11.8867924528301887No Hit
GTGTATAAGCAGATGAAAAATATTTTTTTCTAAAATAATTGCTGGGTGTA11.8867924528301887No Hit
CCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAAGAGGT11.8867924528301887No Hit
ATACTCGGGGGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCGGAGG11.8867924528301887No Hit
ATATTCAACATTCTTCAAGAAAAGAATTTTCAACCCAGAATTTCATATCC11.8867924528301887No Hit
TGCAAGTACAAGCATACCTTGGAGATACCACAGGTTTAGTTTAAGATCAC11.8867924528301887No Hit
CAACAACAATAACCAAAAAAAAAAAAAAAAAAACCAAAACTTGGGAATAA11.8867924528301887No Hit
GATCACTTGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC11.8867924528301887TruSeq Adapter, Index 1 (96% over 25bp)
CGCCTATAATCCCAGTTACTTGGGAGGCTGAGGCAGGAGAATCACTTGAA11.8867924528301887No Hit
CACAAAGACCATCTACCAGTGTTCAGTTCTGGCTCCTCTAATATCCCTTT11.8867924528301887No Hit
GAATTGATGCTAAATGAATGGGTATGCTTTTTTTTTTTAAAAAAAAAAAA11.8867924528301887No Hit
GCACAAGCTTAAACCAGGAGGCAGAGGTTGCAGTGAGCCAAGATCATGCC11.8867924528301887No Hit
CCACTTCACTCCAGCCTGGGCAAAAGAGCAAGCCTCCGTCTCAAAAAAAA11.8867924528301887No Hit
ATATTGCCTGTATTAGACTCAACCTATGCTGGTGTTAGAGAGATGAATTA11.8867924528301887No Hit
GGCTCCCCTTCTTGGGTGTGGAGGGAGACAGTGGTCGGGACCCCCAGAGA11.8867924528301887No Hit
TGTTAAACTAGAGAAGTGTTTTCTCAGTTTAATAATTGCTACCTATACTG11.8867924528301887No Hit
CTTTGGGAGGCTGAGGCAGGAGGATTGCTTGAGCCCAGAAAGTTTGAGAC11.8867924528301887No Hit
GATTAGGTCATGAGTGGAGCCGTAACAAATGAGATTAGTGAACTTGTAGA11.8867924528301887No Hit
ACTCCATATTGTTCTGCAATATTTCGTAAAAAAGCAGTATCTCCTTTTCC11.8867924528301887No Hit
GCCGAGATCACACCATTGCACTTCAGCCTGGGCAGTAAGAGCAAAACTCT11.8867924528301887No Hit
CCTGCCCCCGGATCAAAAATATATATATATTCTTGACTAAAGTCTAGTCA11.8867924528301887No Hit
CCAGCCTCCCTTTGATGGCTTCAAAGAAAGGAAAAGCCAGTGTTCCCAAG11.8867924528301887No Hit
CATCTCTACTAAAAACACAAAAATTAGCTGAGCGTGGTGATGGGTGCCTG11.8867924528301887No Hit
GGCCAACATGGTGGCACCCCATCTCTACTAAAAATACAAAAATTAGCCAG11.8867924528301887No Hit
GTATGGCATATTCTTTAACAAACGCAGAATTTCTAATATTTGCGTTCTTG11.8867924528301887No Hit
GGGAACTGCACCGGGGCACGCATACCAGAGCCCACGAGACGCTCATGAAT11.8867924528301887No Hit
GAACAATAAGTGAATTATTGCACGAAGGACAATCAATTTAAAAGTTTACT11.8867924528301887No Hit
GGTCCCTGCACTACCTCCTGAACAGCCCAATGAGATCATGCCTCATTGTA11.8867924528301887No Hit
GATAGGGCCTTGCTGTGTCACCAGGGTAGAGTGCAGTGATGCAGTCACAG11.8867924528301887No Hit
CAGCAAGACTTTGTCTCAAAAAAAAAAAATGTGGTACTCAATTACAGTTT11.8867924528301887No Hit
GCATTCATGAGATATTTCTCATTACCTGGAGAGCACCCAGACAAGTTCAT11.8867924528301887No Hit
GAGGGAGACTCTGTCTCAAAAAAAAAAAAAGCAACCCTGGCGGCGGCCTC11.8867924528301887No Hit
CCCTTGCCCACCCCGCCCAACTACAATTCTGAATCAAATCAAGCTTCTAG11.8867924528301887No Hit
CTACTAAAAATACAAAAAAATTAGCTGGGCGCGGAGGTGCATGCCTGTAG11.8867924528301887No Hit
ATACAAATCGTACTATTTGTACTGTTATTGATGCTCAGAAAAAGCATGTA11.8867924528301887No Hit
TCCTCATCTGTCACATGATAACAGCATTTACCCCACAGGGTTACTGTGAG11.8867924528301887No Hit
CTTCAAGGGCTTATAATTCAAAACAAAAGAAAACAAAATAAAAAAAAAAA11.8867924528301887No Hit
CCTGTAATCCCAGCACTGTGGGAAGCCGAGGCAGGCATATCACTTGAGGT11.8867924528301887No Hit
CCTTAAAGGCAGGGACTATGTCAGAAATTTATGCCGAGCGCCGTGACTCA11.8867924528301887No Hit
GAAAAGGGTACATAACTAATATCCAAACAGCGTTAATAAAAGCCTATTAA11.8867924528301887No Hit
CCGCCTCAGGCTCCCAAAGTGCTGGGATTACAGGTGCTGTCTCTTATACA11.8867924528301887No Hit
TCTCTATTCAGCTGACTTTTTTTTTTACAAGGGAGCCAACATTATTCCTG11.8867924528301887No Hit
CACCTGTAGTCCCAGCTAGTCTGGAGGCTGAGGCAGGAGAATCACTTGAA11.8867924528301887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACCT50.0145.04
ACACCTG50.0145.05
ACCTGTA50.0145.07
CACCTGT50.0145.06
CTCACAC50.0145.02
TCACACC50.0145.03
GCTCACA50.0145.01
TCCCAGC100.072.5145
CCTGTAA100.072.58
CTGTAAT100.072.59