FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_341.tr1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_341.tr1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7551913
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG48900.017.345685
CTACACT42550.015.6753414
TATACTG66050.014.8175125
GCTACAC33700.014.62925153
CTATACT49900.014.5287484
TCTACAC46250.014.2650313
GTATAGA50200.014.1612411
CTTAGAC31050.013.7763283
GTCTAGA33700.013.7761871
CCTATAC31200.013.4777213
GTATAGG27500.013.4529161
TATACGG16850.013.3375515
GTATAAG45200.013.320441
TAGACAG69850.013.18115
TACACAG59400.013.18115
GTGTAAG38200.013.10281851
TATACCG16650.013.0623515
GTATTAG50900.012.9688821
TCTAGAC34350.012.8749683
GTGTAGG35650.012.8191721
Produced by FastQC (version 0.11.5)