FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_3256.tr4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_3256.tr4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9939845
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA14750.033.913946
CGGAGAT15950.032.2714779
GGGCTCG15750.031.3005354
TCGGAGA17400.028.7488888
GGCTCGG18950.026.7799665
CTCGGAG19800.024.531887
CGTGGGC22600.022.1451261
AGACGCC23400.017.349844145
TATACTG97200.016.1850745
TACACTG79950.015.9593575
CTATACT63900.015.5432954
CTACACT60700.014.8100444
CTTACAC62350.014.6514063
GTGTAGC36850.014.5657131
TACCCCG8101.05501385E-1014.3204335
CTAATAC65950.014.0714993
GTATAGG34200.013.9976581
TACACCG8903.274181E-1113.8477785
TCTACAC61900.013.8209083
GTGTAGG40050.013.5830171
Produced by FastQC (version 0.11.5)