FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_30982.tr4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_30982.tr4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6843104
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG18000.018.1245847
TATACTG54300.017.2233625
TATACCG15950.016.8177975
GCTCGGA20650.016.5008346
GTATAGG24300.015.8210661
CTACACT31750.015.75577454
TACACTG43150.015.2893375
CCTACAC28500.015.2635893
TCGGAGA22500.015.1440978
GTCTAGG23150.015.0402971
TAGTACT29100.014.948334
GTGTAGA42000.014.6803271
TACACCG23600.014.4382295
GTGTAGG30050.014.2420871
TACACAG51150.014.1736755
ATACTGT76100.014.0042796
GGGCTCG24000.013.8957184
GTATTAC36150.013.4441281
CGGAGAT26450.013.4306959
CTAGTAC14950.013.0940173
Produced by FastQC (version 0.11.5)