FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_30900.tr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_30900.tr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTTTGGCTCACATTTATTGAAGAGTTAGAGCTTTTTTTTCAAATCGAT13.225806451612903No Hit
CCTGTAGTCCCAGCTACTTAGGAGGCTGAGGCAGGAGAATTGCTTGAACC13.225806451612903No Hit
CACATATTCAAGTTTGTATCCAGAATTAAGCGAGTCAGTAGTTTTAAATC13.225806451612903No Hit
ACTCCAGCCTGGGCGACAGAGCAAGACTCCGTCTCAAAAAAAAAAAAAAA13.225806451612903No Hit
GAGAACCTCTGTTCTAGGTAAAGAAAGGTAAATTTAGGTGGCAGCTATAA13.225806451612903No Hit
GCATCCTTCTATCTGGAAGCTCCACTTTTCTCTTCAGAACCAGCACTTCT13.225806451612903No Hit
GTGTGATGGTGCATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGG13.225806451612903No Hit
GCAGAAGACCTTTGTTAACTGGTGACAAAGTTAAACAGAAAAACCGATGT13.225806451612903No Hit
ATACAAAAATTACCCAGGAGTGGTGGCACATGCCTGTAGTCCCAGCTACT13.225806451612903No Hit
GCTGGAAGTCAATGCGCATACCCTCATCCTTGGTGAAGCGTCCCGCCTGG13.225806451612903No Hit
CTGTAGTCCCAGCTACTCAGAAGGCTGAGGCAGGAGAATCACTTGAACCC13.225806451612903No Hit
GATGTATGATTTGTAAATATTTTTTCCCATCATTGGGTTTTATTTTCACC13.225806451612903No Hit
GAACCCGGGAGGCGGAGCTTGCAGTGAGCGAAATCGCTCCACTGCACTCC13.225806451612903No Hit
GCTTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAATGCGGTGAAACTCCA13.225806451612903No Hit
CCTCAAGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTT13.225806451612903Illumina PCR Primer Index 2 (96% over 27bp)
GGACTATACTTCTTTTTAGTTTGGCAACACATACTTAACATTTCTTCATT13.225806451612903No Hit
GGCATGGTGGCATGTACCTGTAGTCTCAGCTACTCAGGAGGCTGAGGCAG13.225806451612903No Hit
AGGCCTGACCAGAATTAGAAACCGTGAGAAAGAACCAGAAGGCAATAAAT13.225806451612903No Hit
ACCCTACACAATGCACAGAAAAGACACCCCTCAGACTGAAAGTGCTCTTT13.225806451612903No Hit
CAATTGTTTCAAATGCATTAAAAAAAGGCGGGTTTTGTAGGTCTAAAAAA13.225806451612903No Hit
GATTGAGACCATCCTGGCTAACAAGGTGAAACCTCGTCTCTACTAAAAAT13.225806451612903No Hit
GGAGAATAGCTTGAGCCCAGGGGTTCAAGACCAGCCTGGGAAACATAGTG13.225806451612903No Hit
CCTGTGGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCACTTGAACC13.225806451612903No Hit
CACCACTGTACTCCAGCCTGGGCGACAGAGTGAGACAAGAAAGAAAAAGA13.225806451612903No Hit
GCTAACACGGTGAAACCCTGTCTCTACAGAAAATACAAAAAAATTAGCTA13.225806451612903No Hit
TTTCAGAGCTTGAAGACAGGTTTTAAAAATTCATCCAAATCAGAGAAAAA13.225806451612903No Hit
ATTATATCCTCTGCAAACAAGGATAATTTAACTTCTCTCTTTCCAATTTG13.225806451612903No Hit
GTGACATATTACCTTACCTCAGCCAGAATGGCCATTATTAAAAAGTTAAA13.225806451612903No Hit
GGACTGCTCTTGTCCTATGATGGCAGCAGTTATACTACGTATATGAAAGA13.225806451612903No Hit
ACTCTGGAGGCTGACGCAGGAGAATTGCTTGAACCCAAGAGGCGGAGGTT13.225806451612903No Hit
GGTCAGGACTATGATCCCACCTGAGGCTCAAGGTCTTCTCCAAGCTTATT13.225806451612903No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)