FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n01_MG_30900.tr3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n01_MG_30900.tr3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10624404
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG21900.016.8830095
TATACCG14850.016.5987835
GTGTAAG62200.014.6939681
CCTACAC49250.014.5738133
CTAGACT51100.014.3294114
TAGATCG9203.6379788E-1214.1843185
GTACTAG30300.014.1243441
GTGTAGA69050.013.9716231
GTATAGG41050.013.9595961
TATACTG72550.013.7900395
TACACTG79100.013.747975
CTACACT62550.013.5608354
CTAACAC73300.013.2539423
GTGTAGG51450.012.8296541
TACACAG93350.012.7365945
GTGTATA108300.012.725771
TAGACTG65150.012.5744165
GGACCGT15000.012.5662566
GTATTAG70750.012.5081271
CGTATAC16250.012.4924873
Produced by FastQC (version 0.11.5)