FastQCFastQC Report
Tue 23 Apr 2019
HK75YAFXY_n02_SvSClib2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK75YAFXY_n02_SvSClib2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113810588
Sequences flagged as poor quality0
Sequence length99
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2958680.2599652679063568No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTCGAGTTGGAGCACGC2388970.20990753514075508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT16381050.087.9339141
AGCAGTG17398950.083.6049962
GCAGTGG19369650.075.9982763
CAGTGGT19377350.075.563424
AGTGGTA19820800.074.3049245
GGTATCA19611100.073.502028
GTGGTAT20039600.073.219056
GTATCAA19451100.073.169479
TGGTATC19952850.072.617027
GGTATAA604550.040.703868
GTATCAC675250.039.7773449
ATCAACG19314250.039.2833110-11
CAACGCA19277200.039.15653612-13
ACGCAGA19598550.039.03392814-15
AGAGTAC19875300.038.83727618-19
GCAGAGT20194950.038.51976816-17
TACATGG19732400.038.40419422-23
AGTACAT19914850.038.27862520-21
CATGGGG13775500.037.43579524-25
TCAACGC19280500.036.7762312-13
Produced by FastQC (version 0.11.5)