FastQCFastQC Report
Tue 23 Apr 2019
HK75YAFXY_n01_SvSClib2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK75YAFXY_n01_SvSClib2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113810588
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCTAATCATTGGCTTTACCTGATA2054580.18052626175694655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT582550.019.5997581
GGGAATG789750.019.4345041
CGGAAGC532000.019.262584
ACGGAAG629600.019.1301983
GGAAGCC487300.019.1181435
GACCAAT750800.019.035681
GAAGCCA490700.018.8636826
CATGGCG475800.018.8282531
GGGACCT458250.018.7724951
TGGCCAG654800.018.7505781
TGGTGCA653550.018.7135186
GGAATGG743350.018.7089122
ATGGTGC637100.018.644575
TGCGAGC348200.018.6281434
GCATGCG487150.018.5792711
TGACTAG399400.018.5123181
GGGCATC378800.018.4822581
AAACGGG380850.018.4710061
CACGGAA533200.018.4591272
TACGTCG498950.018.4486685
Produced by FastQC (version 0.11.5)