FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16112915
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT649410.4030369427257575No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA434920.2699201230813915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT62550.010.5772121
CTCGTAT19400.010.102715544
GGGAAAT50050.010.0015821
CGGGGAT62800.09.866211
CGGGCAT34900.09.7293331
GGCGAAT24650.09.65671
TTAGCCG27100.09.5569288
GGGGTAT25550.09.453551
GGGGAAT41500.09.1098751
TATGCCG21750.08.68930448
CGTATTC29450.08.67577524
CGGCTAT23100.08.6377311
AACGTAT27700.08.59237722
GGGAGAT76100.08.5558871
AATACGC28250.08.55005231
GGAAAAT79900.08.4994681
CCCATAT41800.08.4582761
CCGGAAT25350.08.2853341
GCGGGAT36250.08.2081961
GGGAAAC48350.08.1812321
Produced by FastQC (version 0.11.2)