FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13790926
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010880.7330037156315682No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA565400.4099797214487265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT13150.016.76740639
ACCTAGG26150.014.18749710
TATGCCG16500.013.5751848
CTCGTAT17400.013.0742544
TTCCGTA18800.012.10190936
GGCGAAT19650.011.2234191
AATACGC38500.011.00124631
CGCGATA23350.010.9441791
AACGTAT39150.010.90721422
CCTAGGT32500.010.87717111
GCGGAAT21050.010.809571
ATACCTA35400.010.7766128
TATCTCG21850.010.73188641
CGGGCAT27550.010.5463871
CGCTATG21850.010.25250629
CAATACG41500.010.2060530
TACCTAG38550.010.1684379
GGATACC37550.010.1595756
GATACCT36100.010.0828927
ACGTATT45300.09.88984923
Produced by FastQC (version 0.11.2)