FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17283420
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2146181.241756550497529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAT27600.011.7956341
CCGGGAT60850.09.779931
GGGGAAT47650.09.6240041
GGCGAAT26750.09.5531471
GGGAAAT58600.09.3788471
CTCGGAT36050.09.3220921
ACCTAGG22550.09.3125910
CGGGGAT60800.09.0394811
TTAGCCG26350.08.7664318
CGGGCAT35650.08.7393261
CCCGTAT11800.08.6032681
CGCGTAT14300.08.5681
GGGAGAT86000.08.5480741
CGGAAAT27300.08.4630851
CGCGAAT20150.08.3389931
GTCGGAT38300.08.2260471
GGGACAT36200.08.1230321
GTCGAAT29800.08.1055081
TCCCTAC32900.08.0849479
CCGGAAT31400.07.91545961
Produced by FastQC (version 0.11.2)