FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19508361
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3475921.7817591134385917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGC52150.010.6728631
TATGCCG24850.010.56286346
AACGTAT53350.010.36572122
CTCGTAT25100.010.17886342
CAATACG57150.010.04541430
ACGTATT59050.09.77999523
CGGGCAT42000.09.7515721
CGGGTAT17900.09.1914251
CGTATTC62750.09.14759524
CCGGGAT61150.09.1020391
TACGCAA65050.08.93273433
AAACGTA62450.08.91128621
CGCGATA20150.08.8599891
CGCGTAT21050.08.8137721
CGGGACT27250.08.6068931
GCGGAAT26800.08.4901741
GGGAAAC70150.08.48319151
GGGAAAT70000.08.4513621
ACAAACG69750.08.43010519
CAAACGT64850.08.41940620
Produced by FastQC (version 0.11.2)