FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19249234
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1823900.9475182233225488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT26700.011.79736642
TATGCCG28500.011.66631546
AATACGC63700.011.6501831
AACGTAT65750.011.44514722
CAATACG68300.010.76295230
ACGTATT71000.010.50014723
CGTATTC74550.010.14098724
CAAACGT75150.010.0597620
CCGGGAT62050.09.9855551
ACAAACG76450.09.9802619
TACGCAA75100.09.88140233
AAACGTA75750.09.84182521
ATACGCA79250.09.45246432
GGGGTAT28750.09.1319631
ACGCAAA82250.09.10748134
TCGTATG47400.08.8604743
CGCAAAA89650.08.70706135
GGCGAAT27150.08.5097111
GTATTCA94650.08.32037325
CGCGTAT20750.08.2664431
Produced by FastQC (version 0.11.2)