FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24192959
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1038450.42923645677240224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT75350.09.8023331
CGGGGAT101800.09.5593261
GCGGAAT29250.09.2149951
GGGGAAT63100.09.1534511
CGGGCAT58750.09.0566171
CGGGTAT21450.08.9756451
CCGGGAT100150.08.8430041
GCCGGAT81050.08.5946921
GCGGGAT63650.08.5243951
CCCGTAT16850.08.517541
GCCGAAT35300.08.4289691
GGAAAAT117350.08.32245351
GGGGTAT34200.08.0859541
CTACACG45450.08.08586312
GGCGAAT35550.08.0742931
CGCGTAT22150.07.9018091
ACCTAGG28650.07.818455710
TACACGA49450.07.714852313
GGGACAT48000.07.65734671
CCGGAAT43450.07.65357071
Produced by FastQC (version 0.11.2)