FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23894804
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1097780.4594220567785364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT98500.09.8445261
GGGGAAT60450.09.7289121
ACCTAGG29750.09.6469510
GGCGAAT35950.09.5428581
GGGGTAT37700.09.4713121
GCGGAAT29250.09.2154421
GCGGGAT60100.09.0283531
GGGAAAT73200.08.7995011
GGGAGAT113150.08.72460651
GGAAAAT108100.08.6140471
GCCGAAT33650.08.4265781
GTCGATT48000.08.3140941
CGGGGAT99950.08.1956671
GGGACAT44250.08.1484551
ATACCTA38350.08.1223788
CTACACG45750.07.956260712
CGGGTAT20900.07.87232261
GCCGGAT85100.07.77469871
CCCATAT51450.07.75658851
AAAGACG79950.07.704728610
Produced by FastQC (version 0.11.2)