FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24665978
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2408960.9766326719337868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAT40550.011.222621
CCGGGAT76300.010.4604911
CGGGGAT79250.010.2477961
GGGAAAT79950.010.2456431
CGCGAAT27600.09.8929831
CGGGCAT51650.09.6240931
CGGGTAT21350.09.3458491
GGAAAAT127200.08.9991681
GCCGAAT40550.08.8917681
GGCGAAT42550.08.8851751
GGGGAAT70750.08.8071491
CGGCAAT67950.08.70640851
CGGCTAT35650.08.6410191
AATACGC42100.08.23176831
TCCCTAC47900.08.1835629
GGCAAAT96800.07.99205881
GGGAGAT119250.07.74974251
CGGAGAT170400.07.72431561
CGGAAAT39050.07.70937541
GCGGAAT30000.07.7013071
Produced by FastQC (version 0.11.2)