FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13047153
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305480.23413537037543747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA20500.012.2943351
CGGGACT18750.011.574891
AATACGC41200.010.28086331
GCGATAG25150.010.0194622
AACGTAT43500.09.8970122
ACGTATT45950.09.82620223
CCGGGAT36350.09.8225041
TAAGTCG19000.09.7627879
CGCGTAT24650.09.6564711
CAATACG44750.09.54347530
CTCGTAT16350.09.41860242
ACCTAGG25850.09.20689610
TATGCCG17500.09.1995846
CAAACGT47950.09.05106320
ATACCTA28900.08.9615978
TACGCAA48950.08.93903133
AAACGTA49150.08.90176921
ACAAACG49250.08.7412219
ATACGCA50050.08.67272832
CGGGCAT27850.08.6726211
Produced by FastQC (version 0.11.2)