FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22318194
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090260.9365721975532608No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1156500.5181870898693685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31950.015.22625646
CTCGTAT33000.013.999516542
AATACGC94500.011.5943531
ACCTAGG49950.011.56147410
GTATGCC43500.011.18344845
AACGTAT98550.011.08108322
CAATACG102550.010.88899830
CAAACGT103750.010.7613420
ATACCTA60850.010.640658
ACGTATT104300.010.57067423
TATCTCG44850.010.37866139
CCTAGGT57550.010.33887411
GGATACC60000.010.2663816
ACAAACG109300.010.24704119
GATACCT58850.010.2291137
CGTATTC112150.010.20530324
TACGCAA113150.010.05430233
CGCGATA37650.010.041341
AAACGTA108850.010.0003521
CGGGACT34900.09.9298491
Produced by FastQC (version 0.11.2)