FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23100913
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424170.18361611941484737No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA348200.15072997331317597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT102100.010.4571851
CGGGGAT101100.010.2143681
GGGAAAT72800.010.1940131
GGCGAAT30600.010.1814411
CGGGCAT61050.09.97709751
CGGCTAT35400.09.888661
GCGGAAT28000.09.8766521
GGGGAAT60950.09.8211671
GGGGTAT32850.09.5906451
ACCTAGG27150.09.53955810
GGAAAAT104350.09.1917621
CGCGAAT31500.09.1126351
ATACCTA37100.08.9620368
CCGTAAT17250.08.7260971
CGGGTAT18300.08.6079971
GGGAGAT115100.08.5765871
CCCGAAT23850.08.3661791
CCCGTAT14250.08.3522741
GGGATAT51450.08.2326691
CGGAAAT33050.08.1556761
Produced by FastQC (version 0.11.2)