FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16434375
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1435560.8735105533371362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT72900.010.420131
CGCGTAT15550.010.1303181
CGGGGAT66950.010.0390251
GGGAGAT77950.09.6552441
GGGAAAT51150.09.3075251
GGGGTAT24800.09.1749271
CGGGTAT13000.09.1553821
CGGGCAT41400.09.1319671
GGGACAT37900.09.0516511
CGCGATA11650.09.0143881
GGAAAAT77550.08.8473911
TTAGCCG24700.08.7852698
GCGGAAT19900.08.6195361
GGGGAAT41950.08.5950061
GGCGAAT26700.08.5220291
CGCGAAT19150.08.4087211
CGGGGGT44150.08.3252931
ACCTAGG20200.07.970227710
GCCGGAT64400.07.88175771
CGGGACT22750.07.84747031
Produced by FastQC (version 0.11.2)