FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21007954
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8162993.8856663528490207No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376460.17919879299050254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29000.015.6889546
CTCGTAT29850.015.24230442
ACCTAGG40800.012.35262110
AATACGC87700.012.17418131
AACGTAT88900.012.08707822
ACTTAGG37650.011.99391932
CAATACG91500.011.66853130
ACGTATT94850.011.36564323
GTATGCC40100.011.17159445
CGTATTC101150.010.93462424
AAACGTA97850.010.9121
ATACCTA49800.010.8931888
CAAACGT99900.010.72077520
CCTAGGT45700.010.64544211
ACAAACG101800.010.58941819
TACGCAA101700.010.56697633
GGATACC52750.010.4829666
CGCGATA23500.010.4271721
ACGCAAA105850.010.2516534
TACCTAG53900.010.1944459
Produced by FastQC (version 0.11.2)