FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23103461
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3414101.4777439622574298No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA743390.3217656436842947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT36250.011.0088911
CGGGCAT56500.010.594841
GGGAAAT74600.010.5112871
CGGGGAT91450.09.76121
CGGGTAT19900.09.6751181
GGGGTAT33100.09.62411
GGGAGAT112600.09.5754461
TTAGCCG34350.09.4757518
CGGCTAT31850.09.3423521
CCGGGAT87150.09.1582851
CCCGAAT23350.09.1451251
GCCGAAT36800.09.1320891
GGAAAAT107150.08.8863511
GCGGAAT26550.08.5702911
GGGTAAT44650.08.3889771
CCCGGAT49050.08.3501371
CCGGAAT41750.08.3009051
CGGGAAT40000.08.226491
GGGATAT55650.08.1775821
GGGGAAT61750.08.1634221
Produced by FastQC (version 0.11.2)