FastQCFastQC Report
Mon 25 Apr 2016
HK733BGXX_n01_js_dosematrix_a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK733BGXX_n01_js_dosematrix_a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27422887
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3802721.3866957187986808No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA993020.3621135878217345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT57150.010.2291471
CCGGGAT79650.010.1083721
GGCGAAT41050.09.8067221
CGGGGAT82500.09.4621551
GGGGAAT80550.09.0828041
GGGGTAT45050.09.0136841
GCGGAAT32650.09.0060681
CGCGTAT29250.08.9758281
CGGAAAT43350.08.8019031
CGCGATA23600.08.7514331
GGGAGAT130850.08.4805631
GCCGAAT46900.08.4342171
AATACGC66850.08.43080731
CGGGACT33800.08.38894751
GGGAAAT99550.08.29869651
GGGAAAC98250.08.2303551
CGGCAAT71600.08.1647451
GGGACAT58500.08.1380841
GCCAGAT116350.08.1233751
CCCGAAT29800.08.1053541
Produced by FastQC (version 0.11.2)