FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_input_60_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_input_60_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13028072
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15400.022.95322446
CGTATGC16150.021.23757644
ATGCCGT16700.021.16636347
GTATGCC17250.020.69477845
TGCCGTC17100.020.4665848
GCCGTCT18300.018.93340949
CACGGCT27000.013.3517531
GCGGCCT35550.012.3076511
AACGCGG36950.011.7468648
CGGCCTT39850.011.06713612
CCTGCGT39350.011.02905665
TCCTGGC40450.010.9019327
GCTGGCC40500.010.80209639
GCTACAT34100.010.77683635
TCGTATG38700.010.5810343
CCGTCTT34600.010.51970750
GCGTTAT41450.010.47040668
CTCGTAT39850.010.45137242
CGCGGCC43000.010.25679810
TCTCGTA36700.010.20404641
Produced by FastQC (version 0.11.2)