FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_input_300_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_input_300_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14063839
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23600.033.2187548
TGCCGTC24600.031.86873450
GTATGCC24850.031.6885147
CGTATGC25200.031.24850746
ATGCCGT25000.031.21861849
GCCGTCT26500.029.7157751
GTCACGT33900.023.43620929
CGTTTCG35800.021.89940633
CCGTCTT43900.018.41610552
TCTCGTA43700.018.10007943
GTTTCGG45400.017.57718834
AGTCACG45900.017.38528428
CTCGTAT46850.016.8083444
TCGTATG48450.016.54221245
ACGTTTC52400.015.62980232
TCGGAAT53850.015.40360137
ATCTCGT53800.014.96232242
TCACGTT56850.014.40620730
ACGTCTG57850.013.73328315
CACGTTT62350.013.07941631
Produced by FastQC (version 0.11.2)