FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_input_1_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_input_1_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14133565
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT165060.11678582155316085TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29400.041.66477648
CGTATGC30850.039.7063246
TGCCGTC31200.039.3737750
ATGCCGT31650.039.25599749
GTATGCC32150.038.20963747
GCCGTCT32750.037.51014351
GGCCTTA38400.031.89981836
GTCACGT42600.029.16606329
CACGTGG44550.027.65404531
TCACGTG46300.026.83549130
CCGTCTT50050.024.96416752
TCTCGTA50000.024.8489643
AGTCACG52900.023.09026128
TCGTATG54900.022.9498645
CTCGTAT55300.022.6572844
ATCTCGT55700.022.36890242
GTGGCCT57300.021.68390334
GCCTTAT58700.021.52387637
TATCTCG60450.021.01648941
ACGTCTG62700.019.53687115
Produced by FastQC (version 0.11.2)